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Limitations of Ribotyping as Genotyping Method for Corynebacterium ulcerans

Tsuyoshi Sekizuka, Chihiro Katsukawa, Makoto Kuroda, Keigo Shibayama, Ken Otsuji, Mitsumasa Saito, Akihiko Yamamoto, Masaaki Iwaki

EMERGING INFECTIOUS DISEASES（2020）

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Abstract
We conducted molecular typing of a Corynebacterium ulcerans isolate from a woman who died in Japan in 2016. Genomic DNA modification might have affected the isolate's ribotyping profile. Multilocus sequence typing results (sequence type 337) were more accurate. Wholegenome sequencing had greater ability to discriminate lineages at high resolution.
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Key words
Corynebacterium ulcerans,bacteria,genome sequence,molecular typing,multilocus sequence typing,phylogeny,ribotyping,whole-genome sequencing,zoonoses
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