The Determination of Metabolic Pathways of Reishi Polysaccharide in Human Liver Particles and the Vitro Inhibition of CYP450 Enzymes in Human Livers

Reishi Polysaccharide is a three-dimensional monosaccharide chain with a spiral stereotype, and modern pharmacology and clinical trials have confirmed that Reishi polysaccharide plays an important role in the prevention and treatment of various diseases. It has anti-tumor, immunomodulation, anti-radiation, antibacterial, antioxidant and other pharmacological effects. The metabolic pathways and mechanisms of reishi polysaccharides in human liver particles were studied by the in vitro metabolism of human liver particles, and the enzyme dynamics of reishi polysaccharides in human liver particles were discussed. The effect of CYP450 enzyme inhibitors on resinopolygy metabolism is closely related to CYP450 enzyme, which is involved in the metabolism of Reishi polysaccharides in human liver particles. Enrich your knowledge of the main active ingredients. The LC-MS/MS method is established to determine the amount of metabolites corresponding to CYP450 enzyme-specific substrates. To study several aspects of enzyme inhibition evaluation system, optimize and establish a reasonable, accurate and reliable in vitro evaluation system, which can be used to evaluate the in vitro inhibition of CYP450 enzyme in human liver particle body by innovative drugs to predict possible drug interaction in the body. It provides theoretical and experimental basis for a deeper study of pharmacokinetics and clinical rational drug use.