Application of Laser Scanning Confocal Microscope in Ecological Environment

In order to effectively prevent and control the pollution of micron-scale nanoparticles on the ecological environment and protect the home on which human beings depend, in this study, based on laser scanning confocal microscopy (LSCM) technology, the in-loop reflective fluorescence technology (TIRF), fluorescence bleaching recovery technology (TRAP) and LSCM are used. The concept of biofilm is introduced in the ecological environment, especially in the treatment and purification of soil environment. The adsorption, diffusion, solubility and toxicity of micron-scale contaminated nanoparticles on the surface of biofilm are studied. Quasi-zero-dimensional micro-quantum dot nanoparticles with three dimensions less than 100 nanometers are used. Through repeated experiments, it is concluded that the biofilm has a strong adsorption effect on micron-scale CdTe/CdS/ZnS core-shell quantum dot aggregates and can reach the adsorption equilibrium in 25 minutes. At the same time, it is found that the aggregates of micro quantum dots not only adsorbed on the surface of biofilm, but also further diffused into the inside of biofilm. The adsorption index and diffusion index are 1.12*10(-2) and 6.82*10(-3) respectively. Secondly, the micron-scale quantum dot aggregates have a strong toxic effect on the biofilm. Even if nanoparticles form aggregates, they can also enter the biofilm and cause some harm to the ecological environment. Therefore, it is necessary to further study the biofilm in the ecological environment protection. However, this experiment proves that LSCM can be well applied to the ecological environment management, providing technical guarantee for the stable and sustainable development of the ecological environment.