THE PROTECTIVE EFFECT OF THE COMBINATION OF XIAOXIANXIONG DECOCTION AND GUIZHI FULING PILLS ON RAT VASCULAR ENDOTHELIUM POST-MYOCARDIAL INFARCTION

The study aims to explore the potential benefit of Traditional Chinese Medicines on myocardial infarction in well-established rat model. The myocardial infarction rat was established by ligating the left anterior descending coronary arteries. The infarction was evaluated by TTC staining. Traditional Chinese Medicines Xiaoxianxiong decoction and Guizhi Fuling Pills were administrated by gavage. The left ventricular ejection fraction (LVEF) was monitored at week 2, 4 by M-Mode Sonography. The total cholesterol and triglyceride in serum were measured by biochemical auto-analyzer. The change in serous content of C-Reactive Protein (CRP), Homocysteine (Hcy), nitric oxide (NO), endothelin (ET) were determined by ELISA and endothelial nitric oxide synthase (NOS) in myocardial tissue were detected by Western Blot. We found that treatment with the combination of Xiaoxianxiong decoction and Guizhi Fuling Pills could increase the expression level of myocardial eNOS and serous NO concentration, lower the level of CRP, Hcy and ET simultaneously. A significant protective effect was observed in endothelial injury and myocardial infarction. Our results suggested combinational treatment with Xiaoxianxiong decoction and Guizhi Fuling Pills might conserve the vascular endothelium function by promotion the release of relaxing factors, inhibition of the release of contracting factors and reduction of coronary spasm and contraction.