耐药相关果糖二磷酸醛缩酶C的生物质谱分析与鉴定

ACTA CHIMICA SINICA(2006)

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Abstract
醛缩酶C是生命代谢过程中重要糖酵解同工酶之一.应用二维凝胶电泳分离卵巢癌细胞中高表达的果糖二磷酸醛缩酶C,并通过基质辅助激光解吸电离飞行时间串联质谱对其进行分析与鉴定,结果表明应用高分辨二维凝胶电泳分离,结合生物质谱联用技术分析和鉴定未知蛋白具有简单快捷的特点.
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Key words
aldolase C,matrix-assisted laser desorption ionization tandem time of flight mass spectrometry,two dimensional gel electrophoresis
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