Evaluation of Selected SSR Markers for Their Capability to Control the Quality of Cabbage F-1 Hybrids Production

It is clearly evident that F-1 hybrid cultivars are more and more used in commercial production of cabbage. However, produced seeds are often contaminated by seeds with non-hybrid properties. The aim of this work was to evaluate selected SSR and ISSR markers as a potential substitution for traditional field grow-out assessment of F-1 hybrid seeds purity. Individual markers were evaluated on the base of their power to control the quality of seed production and capability to clearly distinguish individual varieties. To simulate standard practice as much as possible, a group of 7 trinities consisted from two parental lines and their crossbred hybrid, all originating from one cooperative seed company, was analysed. Used SSR markers surprisingly offered relatively low degree of polymorphisms. In spite of this fact they were theoretically able to control character of used breeding lines or membership of samples to supposed F-1 variety.