Perfluorosulfonic acid polymer based eATRP for ultrasensitive detection of CYFRA21-1 DNA.
ANALYTICAL METHODS(2020)
摘要
The sensitive detection of biomarker cytokeratin fragment antigen 21-1 (CYFRA21-1) is crucial for early diagnosis and screening of non-small cell lung cancer (NSCLC). In this work, an electrochemical biosensor based on Nafion-initiated eATRP has been built for ultrasensitive detection of CYFRA21-1 DNA for the first time. Specifically, peptide nucleic acid (PNA) probes are immobilized onto a gold electrode surface and then hybridized with target DNA to form PNA/DNA heteroduplexes for the subsequent attachment of Nafion by the identified carboxyl-Zr4+-phosphoric acid chemistry. Finally, polymer chains are obtained by linking the monomer of ferrocenylmethyl methacrylate to the PNA/MCH/DNA/Zr4+/Nafion probesviaeATRP. Under optimized steady-state conditions, the sensor offers a wide current response for CYFRA21-1 DNA from 10(-11)to 10(-16)M with a detection limit of 6.42 x 10(-17)M. The proposed method of using Nafion as the eATRP initiator exhibits high sensitivity, reproducibility and stability and is a promising strategy for early diagnosis of NSCLC.
更多查看译文
关键词
perfluorosulfonic acid polymer,ultrasensitive detection,dna,eatrp
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要