Soil contamination by Taenia solium egg DNA in rural villages in Kongwa district, Tanzania.
Infection ecology & epidemiology(2020)
Abstract
The presence of DNA from eggs in soils around the households in four Tanzanian villages in Kongwa district were analysed in relation to seasonal fluctuations and infection risk implications. A total of 192 pooled soil samples from five sampling points per household were examined by droplet digital Polymerase Chain Reaction (ddPCR) from 96 pig-keeping households both during the dry and rainy seasons. The pooled samples were first processed by a flotation-double sieving technique, followed by screening for worm DNA employing universal primers targeting the mitochondrial cytochrome c oxidase subunit I () gene of human taeniid species and some other helminths. All DNA positive samples were later confirmed by a specific ddPCR probe assay targeting the mitochondrial gene of . A total of 17.2% (n = 33) samples were positive with the universal ddPCR, whereas DNA was confirmed by the specific ddPCR only in 3.1% (n = 3) of the surveyed households. The detection of DNA in this study spells out a low risk of exposure to eggs from contaminated household soil. Based on our results, ddPCR seems to be a promising technology for screening eggs in soil.
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Key words
Kongwa,Taenia solium,Tanzania,ddPCR,eggs,soil contamination
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