Novel microvascular endothelial model utilizing a collagen vitrigel membrane and its advantages for predicting histamine-induced microvascular hyperpermeability

•Collagen vitrigel membrane provides ideal scaffold for cells and pathway of chemicals.•Novel in vitro test system for predicting the microvascular permeability of chemicals.•Extrapolation of drug permeation from inside to outside capillary blood vessels.•High endothelial barrier function with excellent responsiveness to histamine.•Molecular size-selective microvascular hyperpermeability induced by histamine.