Ultimate fate of herbicide tembotrione and its metabolite TCMBA in soil.

Tembotrione is a triketone group herbicide having worldwide applications for weed management in maize. It is considered to be less stable in the environment and its degradation products may have toxicological consequences due to longer persistence and off-site movements. We studied the persistence behavior and leaching potential of tembotrione and its major metabolite TCMBA in clay loam and sandy loam soils having different physico-chemical properties. The rapid transformation of parent tembotrione to degradation products and their high interactions with soil provided challenging task of residues separation from complex soil matrix. Therefore, a novel sample preparation method (modified QuEChERS) was optimized for trace estimation of tembotrione and TCMBA which offered 86.6-95.6% recoveries with limit of detection (LOD) and quantification (LOQ) as 0.001 and 0.003 μg/g, respectively in both soils without any matrix interference. A first order dissipation kinetics was followed by tembotrione and TCMBA residues with half-life ranged from 7.2 to 13.4 days in both soils. Residues reached below detectable limit on 45-60 days after treatments in two application doses. Leaching experiment revealed maximum retention of tembotrione residues from 15 to 25 cm depth in both soils whereas TCMBA show appreciable leaching potential. It was concluded that tembotrione can be phytotoxic to the succeeding crops if applied at late post-emergence stage. TCMBA can contaminate surface and ground water due to continuous and prolonged use of tembotrione particularly in light textured soils.