Direct detection of intact Klebsiella pneumoniae carbapenemase variants from cell lysates: Identification, characterization and clinical implications
Clinical Mass Spectrometry(2020)
Abstract
•LC-MS methods are used to identify intact Klebsiella pneumoniae carbapenemase (KPC).•Mass spectrometry validates the mature form of KPC based on molecular weight.•Combining top-down and bottom-up proteomics confirms mature protein sequence of KPC.•This method can be applied to multiple variants, including KPC-2 through KPC-5.•The method can be easily adapted for KPC determination from any bacterial pathogen.
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Key words
CPO,KPC,CDC,ATCC,MS,MS/MS,LC,MALDI,ESI,TOF,MW,CSD,PCR,BLAST,m/z
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