Blue Light Improves Stomatal Function and Dark-Induced Closure of Rose Leaves ( Rosa x hybrida ) Developed at High Air Humidity.

Plants developed under constant high (>85%) relative air humidity (RH) have larger stomata that are unable to close completely in response to closing stimuli. Roses (Rosa xhybrida) developed in high RH have previously been shown to have high water loss during leaf dehydration and reduced dark-induced closure resulting in a shorter postharvest life. In this study, the effect of B-light on stomatal function under high RH conditions was investigated. The ability of rose leaves developed under continuous high (90%) or moderate (60%) RH to close their stomata in response to darkness and leaf dehydration assay was studied. Moreover, the level and regulation of ABA in light and darkness in relation to B-light was measured. Our results show that increased B-light proportion improved stomatal function and dark-induced stomatal closure under high RH conditions and that was associated with increased [ABA] in general and a dynamic ABA peak during darkness. Furthermore, increased B-light during the day was associated with the presence of high beta-glucosidase activity during night. This indicates that B-light is important as a signal to activate the beta-glucosidase enzyme and release ABA during night. Altogether, the improved stomatal function and reduced transpiration in combination with increased [ABA] indicate that preharvest B-light plays an important role in governing stomatal functionality and ABA homeostasis under high RH and can be a useful method to improve postharvest water balance of roses.