ThruPLEX HV: a simplified system for preparation of molecular-tagged NGS libraries.

We present a complete, fast, modular NGS library prep system that enables accurate, reproducible sequencing readout from highly challenging sample types. The system's features include: 1) a higher input volume to maximize library complexity, 2) tunable enzymatic fragmentation that does not add any time to the workflow, 3) optional molecular tags for detection of low frequency mutations, and 4) optimized conditions throughout to ensure a broad range of accurate GC representation. Performance data is demonstrated for cell line DNA, FFPE DNA, cell-free DNA, and microbial DNA.