The Regulatory Network of CMPG1-V in Wheat- Blumeria graminis f. sp. tritici Interaction Revealed by Temporal Profiling Using RNA-Seq.

Wheat powdery mildew (Pm), caused byBlumeria graminisf. sp.tritici(Bgt), is a prevalent fungal disease. The diploid wheat relativeHaynaldia villosa(H. villosa) showed broad-spectrum resistance (BSR) to Pm. A previous study reported an E3 ligase gene,CMPG1-VfromH. villosa, showing BSR to Pm. To elucidate the regulatory network mediated byCMPG1-V, in this study, gene expression profiling ofCMPG1-Vtransgenic plant (CMPG1-V-OE) and its receptor Yangmai 158 was analyzed and compared afterBgtinoculation at four infection stages. GO and KEGG analysis revealed obvious reprogramming of SA and ABA signaling, starch/sucrose metabolism, and photosynthesis inCMPG1-V-OE, compared with those in Yangmai 158. Transcripts of SA synthesis genesSARD1andUGT, signaling factorsTGAandPRs, andSnRKsin ABA signaling were specifically upregulated inCMPG1-V(OE)rather than Yangmai 158. Transcripts ofLHCIIin photosynthesis,GLUCandTPPin starch/sucrose metabolism were also induced distinctly inCMPG1-V-OE. WGCNA analysis showed crucial regulatory candidates ofCMPG1-V, involving serine/threonine-protein kinase in phosphorylation, glucosyltransferase in flavonoid biosynthesis, defense factor WRKYs, and peroxidase in oxidative stress. Our results facilitate the deciphering of the resistant regulatory network ofCMPG1-Vand the identification of key candidates which might be employed in breeding programs.