Generation of Functional Genetic Study Models in Zebrafish Using CRISPR-Cas9.

Francisco Carmona-Aldana,Hober N Nuñez-Martinez,Carlos A Peralta-Alvarez, Gustavo Tapia-Urzua,Félix Recillas-Targa

CANCER CELL SIGNALING, 3 EDITION: Methods and Protocols（2021）

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摘要

CRISPR-Cas9 is a method for genome editing that can be used efficiently for in vivo applications; the basic implementation of this method is used to generate genome site-directed sequence eliminations. Here we describe a protocol for genome editing using CRISPR-Cas9 in zebrafish (Danio rerio) one-cell embryos.

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关键词

Zebrafish, CRISPR-Cas9, sgRNA, Genome editing, One-cell embryos

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