DNA origami nanostructures as a tool for the targeted destruction of bacteria
Angewandte Chemie International Edition(2019)
摘要
Antibiotic resistance is a growing worldwide human health issue that is now rendering us vulnerable once again to infections that have been treatable for decades. Various approaches have been proposed in an effort to overcome this threat and effectively treat bacterial infections. We use a DNA origami nanostructure, functionalized with aptamers, as a vehicle for delivering the antibacterial peptide lysozyme in a specific and efficient manner, in order to destroy bacterial targets. We test the system against Gram-positive ( Bacillus subtilis ) and Gram - negative ( Escherichia coli ) targets. We use direct stochastic optical reconstruction microscopy (dSTORM) and atomic force microscopy (AFM) to characterize the DNA origami nanostructures and structured illumination microscopy (SIM) to assess the binding of origami to the bacteria. We show that treatment with lysozyme-functionalized origami slows bacterial growth more effectively than treatment with free lysozyme. Our study introduces DNA origami as a tool in the fight against antibiotic resistance, and our results demonstrate the specificity and efficiency of the nanostructure as a drug delivery vehicle.
![Figure][1]
* AFM
: atomic force microscopy
SIM
: structured illumination microscopy
dSTORM
: direct stochastic optical reconstruction microscopy
[1]: pending:yes
更多查看译文
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要