Deinococcus Terrestris Sp. Nov., A Gamma Ray- And Ultraviolet-Resistant Bacterium Isolated From Soil

Strain SDU3-2(T) was isolated from a soil sample collected in Shandong Province. PR China. Cells of SDU3-2(T) were spherical. Gram-stain-positive, aerobic and non-motile. Cellular growth of the strain occurred at 25-45 degrees C, pH 5.5-8.5 and with 0-1.5% (w/v) of NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain SDU3-2(T) was closest to the type strain Deinococcus murrayi ALT-1b(T) with a similarity of 95.2%. The draft genome was 3.49 Mbp long with 69.2 mol% G+C content. Strain SDU3-2(T) exhibited high resistance to gamma radiation (D-10 >12 kGy) and UV (D-10 >900J m(-2)). The strain encoded many genes for resistance to radiation and oxidative stress, which were highly conserved with other Deinococcus species, but possessed interspecific properties. The major fatty acids of SDU3-2(T) cells were C-15:1 omega 6c, C-16:1 omega 7c/C-16:1 omega 6c, and C-17:1 omega 8c, the major menaquinone was menaquinone-8, and the major polar lipids were an unidentified phosphoglycolipid, four unidentified glycolipids and an unidentified phospholipid. The average nucleotide identity and DNA-DNA hybridization results further indicated that strain SDU3-2(T) represents a new species in the genus Deinococcus, for which the name Deinococcus terrestris sp. nov. is proposed. The type strain is SDU3-2(T) (=CGMCC 1.17147(T)=KCTC 43098(T)).