Crystallographic and cryogenic electron microscopic structures and enzymatic characterization of sulfur oxygenase reductase from Sulfurisphaera tokodaii

•Sulfur oxygenase reductase (SOR) was biochemically and structurally characterized.•High resolution structures of SOR were determined by crystallography and cryo-EM.•Twenty-four identical subunits of SOR form a hollow sphere.•Catalytic components exhibited different features in the crystal and cryo-EM structures.