Toxicity assessment of marjoram and pomegranate aqueous extracts for Cobb chicken, non-target organisms of pest control

This research aimed to evaluate the effect of 5% aqueous extracts of marjoram ( Origanum majorana L.) and pomegranate ( Punica granatum L.) on Cobb chicken ( Gallus gallus domesticus L.), as a model to birds as non-target organisms for pest control. The extracts were prepared using dried ground leaves. The powder obtained was then added to distilled water to obtain 5% extract (w/v). After 48 h, the plant extracts were filtered and added to the feed of 36 female birds. For that, the chicken (12 days old) were acclimated for 3 days before starting the experiment. The plant extracts were administered for 6 days, always preceded by at least 14-h starvation. The birds were evaluated for 11 days, assessing behaviour, feed consumption and animal performance. After sacrificing the birds, histopathological examination was performed, and intestinal villi were measured. No death nor any alteration occurred during the experiment. There was no statistical difference among the treatments regarding feed consumption and performance even though there was intestinal villi reduction.