Role of ClpB From Corynebacterium crenatum in Thermal Stress and Arginine Fermentation.

ClpB, an ATP-dependent molecular chaperone, is involved in metabolic pathways and plays important roles in microorganisms under stress conditions. Metabolic pathways and stress resistance are important characteristics of industrially -relevant bacteria during fermentation. Nevertheless, ClpB-related observations have been rarely reported in industrially -relevant microorganisms. Herein, we found a homolog of ClpB fromCorynebacterium crenatum. The amino acid sequence of ClpB was analyzed, and the recombinant ClpB protein was purified and characterized. The full function of ClpB requires DnaK as chaperone protein. For this reason,dnaK/clpBdeletion mutants and the complemented strains were constructed to investigate the role of ClpB. The results showed that DnaK/ClpB is not essential for the survival ofC. crenatumMT under pH and alcohol stresses. The ClpB-deficient or DnaK-deficientC. crenatummutants showed weakened growth during thermal stress. In addition, the results demonstrated that deletion of theclpBgene affected glucose consumption andL-arginine,L-glutamate, and lactate production during fermentation.