Adenosine monophosphate deaminase 3 null mutation causes reduction of naive T cells in mouse peripheral blood
BLOOD ADVANCES(2020)
Abstract
Adenosine monophosphate deaminase 3 (Ampd3) encodes the erythrocyte isoform of the adenosine monophosphate (AMP) deaminase gene family. Mutations in this gene have been reported in humans, leading to autosomal-recessive erythrocyte AMP deaminase deficiency. However, the mutation is considered clinically asymptomatic. Using N-ethyl-N-nitrosourea mutagenesis to find mutations that affect peripheral lymphocyte populations, we identified 5 Ampd3 mutations (Ampd3(guangdong), Ampd3(carson), Ampd3(penasco), Ampd3(taos), and Ampd3(commanche)) that strongly correlated with a reduction in naive CD4(+) T and naive CD8(+) T-cell populations. Causation was confirmed by targeted ablation of Ampd3. Knockout mice had reduced frequencies of CD62L(hi)CD44(lo) CD4(+) naive and CD8(+) naive T cells. Interestingly, these phenotypes were restricted to T cells circulating in peripheral blood and were not seen in T cells from secondary lymphoid organs (lymph nodes and spleen). We found that reduction of naive T cells in the peripheral blood of Ampd3(-/-) mice was caused by T-cell-extrinsic factor(s), which we hypothesize to be elevated levels of adenosine triphosphate released by Ampd3-deficient erythrocytes. These findings provide an example in which disruption of an erythrocyte-specific protein can affect the physiological status of lymphocytes in peripheral blood.
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Key words
monophosphate deaminase,adenosine,null mutation,cells
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