Disposable Stochastic Sensors Based on Nanolayer Deposition(s) of Silver and AgC Composite on Plastic for the Assay of α-amylase in Whole Blood and Saliva.

Nanolayer deposition(s) of Ag and AgC composite on a plastic substrate was used to design disposable stochastic sensors. Three shapes of nanocoatings were tested. The first shape was obtained by deposition of a nanofilm of Ag on the plastic material; the second shape was obtained by deposition of a composite AgC nanolayer on the plastic material; the third shape was obtained by nanolayer deposition of a composite material of AgC on the top of the Ag nanofilm deposited on the plastic material. alpha-Cyclodextrin was used to modify the active surface of the sensor. Wide linear concentration ranges were obtained as follows: for de-assay of alpha-amylase in whole blood: 1.00 x 10(-7)-1.00 x 10(3)U mL(-1)and for the assay of alpha-amylase in saliva: 1.5 x 10(-15)-1.5 x 10(2)U mL(-1). alpha-Amylase was reliably determined from whole blood and saliva samples using the proposed disposable stochastic sensors.