TILRR Promotes Migration of Immune Cells Through Induction of Soluble Inflammatory Mediators.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY(2020)
摘要
TILRR has been identified as an important modulator of inflammatory responses. It is associated with NF-kappa B activation, and inflammation. Our previous study showed that TILRR significantly increased the expression of many innate immune responsive genes and increased the production of several pro-inflammatory cytokines/chemokines by cervical epithelial cells. In this study, we evaluated the effect of TILRR-induced pro-inflammatory cytokines/chemokines on the migration of immune cells. The effect of culture supernatants of TILRR-overexpressed cervical epithelial cells on the migration of THP-1 monocytes and MOLT-4 T-lymphocytes was evaluated using Transwell assay and a novel microfluidic device. We showed that the culture supernatants of TILRR-overexpressed HeLa cells attracted significantly more THP-1 cells (11-40%,p= 0.0004-0.0373) and MOLT-4 cells (14-17%,p= 0.0010-0.0225) than that of controls. The microfluidic device-recorded image analysis showed that significantly higher amount with longer mean cell migration distance of THP-1 (p< 0.0001-0.0180) and MOLT-4 (p< 0.0001-0.0025) cells was observed toward the supernatants of TILRR-overexpressed cervical epithelial cells compared to that of the controls. Thus, the cytokines/chemokines secreted by the TILRR-overexpressed cervical epithelial cells attracted immune cells, such as monocytes and T cells, and may potentially influence immune cell infiltration in tissues.
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关键词
TILRR,pro-inflammatory cytokines,chemokines,cervical epithelial cell culture supernatants,THP-1,MOLT-4,Transwell assay,microfluidic device
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