Characterization and improvement of curdlan produced by a high-yield mutant of Agrobacterium sp. ATCC 31749 based on whole-genome analysis.
Carbohydrate Polymers(2020)
摘要
•The curdlan biosynthesis mechanism can be determined based on whole-genome analysis.•Mutant CGMCC 11546 produced 1.34-fold more curdlan than the WT strain.•The maximum curdlan production was 47.97 ± 0.57 g/L (16 % increase) after optimization.•Deletion of exoK and exsH increased the gel strength and molecular weight of curdlan.•Noodles supplemented with curdlan from ΔexoK-exsH had excellent eating quality.
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关键词
Curdlan,Agrobacterium,Whole-genome analysis,Gel strength,exoK,exsH,Noodle production
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