硫酸-紫外法与苯酚-硫酸法测定千两茶中总多糖的比较
中国实验方剂学杂志(2014)
Abstract
目的:比较苯酚-硫酸法与硫酸-紫外法在测定千两茶多糖含量上的差异.方法:千两茶粉经水提醇沉,Sevage法除蛋白后,采用苯酚-硫酸法和硫酸-紫外法测定茶多糖含量.结果:苯酚-硫酸法标准曲线方程为Y=4.7550X-0.0295(R2=0.990 4),硫酸-紫外法标准曲线方程为Y=4.7700X-0.065 I(R2 =0.993 1),均在0.02 ~0.1 g·L-1有良好的线性关系.所测茶多糖质量分数分别为2.00%,3.21%,其差异有统计学意义.结论:硫酸-紫外法作为一种多糖检测新手段,可简便测定多糖含量.
MoreKey words
tea polysaccharides,qianliang tea,phenol-sulfuric acid,sulfuric acid-UV,determination
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