Gordonia Mangrovi Sp. Nov., A Novel Actinobacterium Isolated From Mangrove Soil. In Hainan

A novel actinobacterium, designated strain HNM0687(T), was isolated from mangrove soil samples collected from Hainan Province, PR China and its polyphasic taxonomy was studied. Based on the results of 165 rRNA gene sequence analysis, strain HNM0687(T) was closely related to Gordonia bronchialis NBRC 16047(T) (98.7%), Gordonia rhizosphera NBRC 16068(T) (98.2%), Gordonia oryzae RS15-1S(T) (97.9%), Gordonia polyisoprenivorans NBRC 16320(T) (97.7%) and Gordonia sediminis AMA 120(T) (97.7%). Genome-based comparisons revealed a clear distinction in average nucleotide identity values between strain HNM0687(T) and its closely related strains (74.4-78.3%). Strain HNM0687(T) contained meso-diaminopimelic acid, arabinose and galactose in wholecell hydrolysates. Mycolic acid was present. The menaquinones of strain HNM0687(T) were MK-9(H-2) and MK-7(H-2). The phospholipids of the isolate were composed of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids were C-16:0, C-16:1,omega 7c/C-16:1 omega 6c, C-18:0 10-methyl (TBSA), C-18:0 and C-18:(1)omega 9c. Based on its genotypic, chemotaxonomic and phenotypic characteristics, it is concluded that strain HNM0687(T) represents a novel species of the genus Gordonia for which the name Gordonia mangrovi sp. nov. is proposed. The type strain is HNM0687(T) (=CCTCC AA 2019074(T)=KCIC 49383(T)).