Monitoring antibody response following SARS-CoV-2 infection: diagnostic efficiency of 4 automated immunoassays

Introduction: SARS-CoV-2 seroconversion is important for epidemiological studies as well as contact tracing.Material and methods: The antibody response against SARS-CoV-2 was examined in 111 patients with a positive qRT-PCR. Seroconversion was assessed using the Elecsys from Roche, the Liaison S1/S2 IgG fromDiasorin, the IgG and IgA fromEuroimmun, aswell as the VIDAS IgG and IgM. Specificitywas estimated based on themeasurement of SARS-CoV-2 antibodies in 96 residual samples collected during a non-pandemic period.Results: The highest overall sensitivity for detecting seroconversion was obtained using the Elecsys (81.1%), the Euroimmun with a combined detection of IgG/IgA (86.5%), and the VIDAS with a simultaneous measurement of IgG/IgM(78.4%).The Elecsys and the VIDAS IgG/IgMdemonstrated a specificity as well as a positive predictive value of 100%.Conclusions: The Elecsys and the VIDAS methods with a combination of IgG/IgM measurement demonstrated a high sensitivity with no false positive results. (C) 2020 Elsevier Inc. All rights reserved.