Effect of endotoxin on coagulation , fibrinolysis , kinin forming and complement systems in Japanese monkeys ( M . fuscata fuscata )

Endotoxinemia was produced in six Japanese monkeys (7-13 kg) by two suitably spaced (24 hrs, Fig. 1, Experiment I) and by a continuous (8 hrs, Fig. 1, Experiment II) intravascular injection with endotoxin (ET) from E. coli. The changes of concentration of ET in plasma, and faculties of coagulative, fibrinolytic, kinin forming and complement systems were investigated before and after treatment with ET. Body temperature, arterial pressure, pH, p02, pC02, Ht and Hb in blood were checked throughout the experiment. The main observations were as follows. [1] Two spaced injection of ET (0. 3-2 mg/kg, 3 monkeys) Et in plasma rose to 0. 1-1pg/ml of plasma immediately after the injection of ET and thereafter decreased gradually (Fig. 1, Experiment I). Activation of coagulation system was suggested by 60 to 70% decrease in platelet count and 20 to 30% prolongation of APTT and PT except for TT. Intrinsic coagulation factors, F. XII, F. XI, F. IX, F. VIII and extrinsic factor, F. VII decreased 20