Microbiota modification with probiotics induces hepatic bile acid synthesis via downregulation of Fxr/Fgf15 axis in mice” Authors:

SUPPLEMENTAL INFORMATION SUPPLEMENTAL EXPERIMENTAL PROCEDURES VSL#3 protocol administration The probiotic mixture VSL#3 (VSL Pharmaceuticals Inc., Gaithersburg, MD) consists of eight live, lyophilized bacterial strains including four strains of Lactobacilli (L. paracasei (DSM24733), L. plantarum (DSM24730), L. acidophilus (DSM24735) and L. delbrueckii subspecies L. bulgaricus (DSM24734)), three strains of Bifidobacteria (B. longum (DSM24736), B. breve (DSM24732) and B. infantis (DSM24737)) and Streptococcus thermophilus (DSM24731). VSL#3 mixture was freshly prepared every day by dissolving 50x10 9 colony-forming units (cfu) in a total volume of 200μl of saline.