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Cardiovascular diseases (CVDs) are the main cause of death in the world and in Europe. Although in vitro diagnostic (IVD) for Acute Myocardial Infarction (AMI) relies on well-established biomarkers, it is evident the need of a diagnostic platform combining distinct biomarkers which would provide a more complete information of the progression of the disease, the prognosis or a more accurate stratification of the patients to provide a more personalized medicine. Ongoing clinical studies have proposed different biomarkers for a wide monitoring of different cardiovascular diseases, from early stages such as inflammation to heart failure. Accordingly from these studies, cardiac Troponin I (cTnI), C-reactive protein (CRP), N-terminal pro-Brain Natriuretic Peptide (NTproBNP), Cystatin C (CysC) and Heart Fatty Acid Binding Protein (HFABP) have been identified as priority biomarkers to assist clinicians in this respect and therefore for a better diagnosis and prognosis of CVDs. Antibodies for cTnI and NTproBNP produced and characterized in our group and other commercial immunoreagents for CRP, Cystatin C and HFABP, have been combined and used to develop a multiplexed microarray device able to analyze simultaneously these biomarkers in plasma and serum samples. Protein microarrays provide high analytical resolution, detection sensitivities and sample throughput. References: 1. Chan, D.; Ng, L. BMC Medicine 2010, 8, 34 2. Friess, U.; Stark, M. Analytical and Bioanalytical Chemistry 2009, 393, 1453-1462 3. Cameron, S. J.; Sokoll, L. J.; Laterza, O. F.; Shah, S.; Green, G. B. Clinica Chimica Acta 2007, 376, 168-173 4. James, S. K.; Lindahl, B.; Siegbahn, A.; Stridsberg, M.; Venge, P.; Armstrong, P.; Barnathan, E. S.; Califf, R.; Topol, E. J.; Simoons, M. L.; Wallentin, L. Circulation 2003, 108, 275-281 5. Kilcullen, N.; Viswanathan, K.; Das, R.; Morrell, C.; Farrin, A.; Barth, J. H.; Hall, A. S. Journal of the American College of Cardiology 2007, 50, 2061-2069 6. Zethelius, B.; Berglund, L.; Sundström, J.; Ingelsson, E.; Basu, S.; Larsson, A.; Venge, P.; Ärnlöv, J. New England Journal of Medicine 2008, 358, 2107-2116