Novel reassortant human-like H 3 N 2 and H 3 N 1 influenza A viruses detected in pigs 1 are virulent and antigenically distinct from endemic viruses 2

1 are virulent and antigenically distinct from endemic viruses 2 Daniela S. Rajão, Phillip C. Gauger, Tavis K. Anderson, Nicola S. Lewis, Eugenio J. 3 Abente, Mary Lea Killian, Daniel R. Perez, Troy C. Sutton*, Jianqiang Zhang, Amy 4 L. Vincent# 5 6 Virus and Prion Diseases Research Unit, National Animal Disease Center, ARS-USDA, 7 Ames, Iowa, USA; Department of Veterinary Diagnostic and Production Animal 8 Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, USA; 9 Department of Zoology, University of Cambridge, Cambridge, United Kingdom; 10 Diagnostic Virology Laboratory, National Veterinary Services Laboratories, Science, 11 Technology and Analysis Services, Veterinary Services, APHIS-USDA, Ames, Iowa, 12 USA; Poultry Diagnostic and Research Center, University of Georgia, Athens, Georgia, 13 USA; Virginia-Maryland College of Veterinary Medicine, Department of Veterinary 14 Medicine, University of Maryland, College Park, Maryland, USA. 15 16 Running title: Novel Human-Like H3 Influenza A Viruses in Pigs 17 18 Abstract word count: 248; Text word count: 5277 19 #Address correspondence to Amy L. Vincent, amy.vincent@ars.usda.gov 20 *Present address: Troy C. Sutton, National Institute of Allergy and InfectiousDiseases, 21 National Institutes of Health, Bethesda, Maryland, USA. 22 JVI Accepted Manuscript Posted Online 26 August 2015 J. Virol. doi:10.1128/JVI.01675-15 Copyright © 2015, American Society for Microbiology. All Rights Reserved.