Genetic Engineering and Metabolite Profiling for Overproduction of 4 Polyhydroxybutyrate in Cyanobacteria

Running title: Bioplastic Overproduction in Cyanobacteria 1 2 3 Genetic Engineering and Metabolite Profiling for Overproduction of 4 Polyhydroxybutyrate in Cyanobacteria 5 6 7 Sayaka Hondo, Masatoshi Takahashi, Takashi Osanai, Mami Matsuda, 2,4 8 Tomohisa Hasunuma, Akio Tazuke, Yoichi Nakahira, Shigeru Chonan, Morifumi 9 Hasegawa, and Munehiko Asayama ,* 10 11 12 College of Agriculture, Ibaraki University, 3-21-1 Ami, Ibaraki 300-0393, Japan 13 Japan Science and Technology Agency (JST), Precursory Research for Embryonic 14 Science and Technology (PRESTO), 4-1-8 Honcho Kawaguchi, Saitama 332-0012, 15 Japan 16 3 RIKEN Genomic Sciences Center, 1-7-22 Suehiro-cho, Yokohama 230-0045, Japan 17 Organization of Advanced Science and Technology, Kobe University, 1-1 Rokkodai, 18 Kobe 657-8501, Japan 19 20 21 To whom correspondence should be addressed. Tel / Fax: +81-29-888-8651; E-mail: 22 asam@mx.ibaraki.ac.jp 23 24 Abbreviations: CE-MS, capillary electrophoresis-mass spectrometry; NMR, 25 nuclear magnetic resonance; PHA, polyhydroxyalkanoate; PHB, polyhydroxybutyrate; 26 TEM, transmission electron microscope 27 28