Crystal structure of taxuspinanane A acetonitrile solvate , C 47 H 59 NO 14 · CH 3

Source of material Mixture of taxanes was isolated by extraction of the stems of Taxus canadensis with methanol. Pure taxuspinanane A was obtained by a combination of chromatography on a silica gel (Merck) using the mixture of ethyl acetate/toluene (4:6, v/v), and preparative chromatography on a column (21.2 χ 250 mm, I.D.) filled with a silica gel (Phenomenex, Luna Silica, 5 μια) using the mixture of 4-methyl-2-pentanone/toluene (4:6, v/v). Identity of sample was verified by MS and NMR according to published data [1]. Single crystals of taxuspinanane A were obtained by the slow evaporation of its solution in acetonitrile (30 mg/3 ml) at ambient temperature (298 K).