and osteogenic di ff erentiation of MC 3 T 3E 1 cells on Ti 6 Al 4 V alloys modi fi ed with reduced graphene oxide †

Graphene and its derivatives, including graphene oxide (GO) and reduced graphene oxide (rGO), have been considered as promising candidates in tissue regeneration. However, there is little information about their use as dental implant coating materials. In the present study, we fabricated an rGO coating on Ti6Al4V alloys. Then, the effect of the rGO coating on the growth and osteogenic differentiation of MC3T3-E1 cells was investigated. Cell culture results showed that the rGO coating apparently stimulated cell adhesion and proliferation, and enhanced the growth and osteogenic differentiation of MC3T3-E1 cells on Ti6Al4V implants. Our studies demonstrate that the rGO-Ti substrate appears to be biocompatible and has osteoinductive potential, which suggests that rGO might be a potential coating material for promoting the osteogenic properties of titanium alloy dental implants.