Exploring the potential of the last generation UHR-Q-TOF for rapid generation of accurate information on proteoforms distribution and relative abundancy

All along their life cycle, proteins undergo various transformations that can alter their functions while keeping a good part of their primary sequence intact. These multiplication of PTM patterns, alternative splicing forms or products of proteolitic processing cannot be simply resolved with a bottom-up approach, as very few peptides are specific from the given proteoform. However, the information relative to the distribution of different proteoforms is encoded in their intact masse. Being able to catch this information efficiently will drive the biologist into a new analytical dimension, far beyond the traditional gel-based approaches.