Antioxidant and anti tumoral activities of hydrazylpyrrolidine 2, 5 dione substituted and 2- thioxo

In continuation of our research efforts to evaluate biological properties of synthesized compounds, we have investigated the in vitro antioxidant activity of hydrazylpyrrolidine 2, 5 dione substituted 2, 3and4or 2-thioxo imidazolidine 4-one5, 6and7 by spectrophotometric DPPH and lipid peroxide inhibition. The IC50 of both compounds was higher excepted compounds 6b, 7a and 4c witch have a low value (4.9, 6.18, 6.70 μg/mL) respectively.Thesecompounds exhibited strong antioxidant activity. Their IC50 are approximatively equal to standard quercetin for 4c and 7a or to ascorbic acid standard for 6b. In vitro lipid peroxydation was determined by antioxidant activity using the β-Carotene/ linoleic acid system. The synthesis compounds have revealed a low or moderate antioxidant activity. The maximum relative antioxidant activity (AAR) was observed for derivatives 2a and 7c respectively (53.31, 48.72 μg/mL). In vitro antitumoral activities of compounds 2a and 6b at 370 μg/mLhave exhibited significant antitumoral activities against HEp2 (Human Laryngeal CarcinomaCells).