Evaluation of hepatoprotective activity of aya bringaraja karpam , a herbo metallic siddha formulation by using zebra fish model

Liver plays a vital role in detoxifying toxins from the blood, restoring energy and nutrients, digesting fats and processing food and medications in our body.Genetic factors, obesity, viruses, alcohol consumption and chronic usage of medications such as Anti-psychotic drugs, Analgesics, Anabolic steroids and oral contraceptives are some of the common factors causing liver diseases. Untreated liver diseases may also results in Liver Cirrhosis which is a life threatening condition. In Siddha system of medicine, there are numerous formulations with hepato protective activity. Aya Bringaraja Karpam is a popular and unique Siddha formulation with rejuvenative property commonly used in the management of Iron deficiency anemia, Jaundice and greying of hair. Aim The study is aimed to assess the hepato protective activity of Aya Bringaraja Karpam (ABK). Materials and Method The study drug purchased from SKM Siddha and Ayurvedha Pharmacy, Erode, Tamilnadu and hepato protective activity study was performed by paracetamol induced Liver dysfunction in Zebra fish Danio rerio model. The study was conducted in 4 groups with 10 adult Zebra fish each. Group I was treated as control, Group II treated as disease control group, Group III and IV received test drug ABK at the low and high doses respectively. Histo pathological assessment was done on the liver specimens of Zebra fish after 7 days exposure period. Results :The result revealed that paracetamol treated groups shows severe liver degeneration whereas treatment with test drug ABK at both the dose level of 250 and 500 mg/liter significantly attenuated the paracetamol induced Hepatotoxicity. This present study confirms the hepato protective effect of Aya Bringaraja Karpam against paracetamol induced liver damage in zebra fish model and it can be used for the management of liver diseases.