Somatic embryogenesis and plantlet regeneration of Mangifera persiciforma

A method for somatic embryogenesis and plant regeneration from immature nucellus (30–40 days old after pollination) of Mangifera persiciforma C.Y. Wu & T.L. Ming has been developed. Browning and necrosis of the nucellar explants were effectively controlled by pre-treating in an antioxidant solution and by pre-culturing in liquid medium in a constant temperature shaker. The explants were cultured for 2 weeks in the dark at 23 ± 2°C on induction medium and then moved to the light for 4 weeks at 25 ± 2°C Pro-embryonic masses have gradually been generated from the explants. The pro-embryogenic masses were subcultured on appropriate proliferation medium in the light for 5 weeks at 25 ± 2°C, secondary embryogenesis and organogenesis was observed. Entire plantlets have been produced. Plantlets developed in 3-4 weeks, were acclimatized and transplanted to the greenhouse. Morphogenic competence was sustained for more than one year.