Biomarkers as Bioindicators to Early Detection of Pollution Effects in Environmental and the Human Health

The aim of this research was to analyze biochemical parameters in two fish species (Chondrostoma nasus) and (Salmo trutta m. fario) taken in two different rivers of Kosovo. Fish samples were collected during March-July-October 2018 applying the electrofishing method (according to Hans Grassl GmbH) alongside Llapi (samples were taken near to Sitnica river) and Lepenci rivers. The biochemical parameters: plasma glucose (GLU), total protein (TP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were analyzed in the blood samples of 60 fishes of both species. GLU was analyzed by Glucose-Oxidase Enzymatic method, while determination and concentration of TP was analyzed with Biuret Method. Meanwhile enzymes analysis, AST and ALT respectively were conducted according the Enzymatic-Colorimetric method, with kit (Roche) and analyzer Cobas Integra 400-Roche. The research results showed a significant (P<0.05 and P<0.01) increase in GLU concentration in two fish species taken in the Llapi and Lepenci river compared with the normal values, while a significant decrease (P<0.05 and P<0.01) in TP concentration. There is also a highly significant increase in the AST and ALT levels in the blood of fish species (P<0.05 and P<0.01) compared to the normal values for. Statistical data were calculated One-way ANOVA using Minitab software. Experimental article (J Int Dent Med Res 2020; 13(1): 8-16)