GSH Conjugate Screening with the New QTRAP ® 5500 LC / MS / MS System

Screening for reactive metabolites of drug candidates using GSH trapping is an important part of early safety assessment in pharmaceutical discovery and development. Due to the toxicity of reactive metabolites, detection of these species even at trace levels can be relevant in the optimal design of a therapeutic drug. Current low and high-resolution single MS based detection can often be insufficient in terms of sensitivity of detection and confirmation. The QTRAP 5500 system is the first LCMS system which can combine the most powerful modes of GSH metabolite detection into a single injection workflow with sufficient sensitivity to ensure an accurate result. A series of groundbreaking workflows with optimization of scan speed, quadrupole MS/MS sensitivity, rapid positive/negative switching and unprecedented LIT MS/MS confirmation sensitivity will be presented in this application overview.