NoteMeasurement of Gly m Bd 30 K , a Major Sandwich Enzyme-linked ImmunosorbentSoybeanAssayAllergen , in Soybean Products by a

derrnatitis, we found 16 allergenic proteins in the soybean. i) About two-thirds of soybean-sensitive patients examined was shown to have IgE antibodies binding to the 30-kDa protein narned Gly m Bd 30K. This finding suggests that Gly m Bd 30K is a major allergen in the soybean and that it may be a target allergen for hypoallergenization of soybean products. Gly m Bd 30K has been purified to homogeneity and shown to be identical with the 34-kDa oil body-associated protein,2'5) For the development of hypoallergenic soybean products, it is essential to establish a