Hepatoprotective Effects of Zamzam water against Carbon Tetrachloride Induced Liver Damage in Rats : Biochemical ,

Alkaline water have been claimed to boost heptaorotective effect. Thus, this study aimed to investigate if Zamazam water (ZW) that is similarly alkaline, can promote hepatoprotective effect. Hepatoprotective properties of ZW were investigated in a rat model of liver injury induced by carbon tetrachloride (CCl4). ZW is alkaline natural water which makes it potentially capable of enhancing antioxidant power. In this study we investigated the efficacy of the hepatoprotective activity of ZW against carbon tetrachloride induced liver intoxication in Wister albino rats. Carbon tetrachloride was used as a hepatotoxic agent while, ZW were used as a probable hepatoprotective agent. 24 rats were divided into four main groups. Group I, served as normal control, Group II served as liver injured group treated only with CCl4, Group III served as ZW control, and Group IV served as liver overcome group, treated with CCl4 and drinking ZW. Various biochemical parameters supported by histopathology of liver sections were studied to evaluate the hepatoprotective activity of ZW. The study was also supported for the first time in case of ZW evaluation effects by using of DNA extraction of the rats’ livers to investigate the genomic DNA integrity. Results revealed that the serum biomarkers in carbon tetrachloride treated rats recorded elevated concentration indicating severe hepatic damage by carbon tetrachloride. The results of the serum biomarkers of ZW treated rats showed significant reduction indicating the effect of ZW in restoring the normal structural and functional ability of the hepatocytes. Both the molecular and histopathological result showed protective effect in the experimental model of hepatic alterations of rats, and suggests the use of ZW as a hepatoprotector agent in the diet of patients with hepatopathies. [Saif A, Sarhan O, Elmogy M, Mutwally H. Hepatoprotective Effects of Zamzam water against Carbon Tetrachloride Induced Liver Damage in Rats: Biochemical, Histopathological, and molecular Evidences. Life Sci J 2014; 11(10):300-308] (ISSN: 1097-8135). http://www.lifesciencesite.com. 43