Highly Expressed Ddx10 Promotes Hepatocellular Carcinoma Cell Proliferation Through Wnt/Beta-Catenin Signaling

DDX10, a putative DEAD-box RNA helicase gene, is poorly studied in cancer. Our previous study found that DDX10 was significantly up-regulated in hepatocellular carcinoma (HCC). However, the clinical significance of DDX10 and its biological roles and associated mechanisms in HCC tumorigenesis remain elusive. In current study, quantitative real-time PCR, Western bolt were applied to evaluate the expression of DDX10. The roles of DDX10 in cell viability and proliferation were analyzed by cell biological assays in vitro. Luciferase reporter assays was employed to investigate the mechanism. And we further confirmed that compared with adjacent tissues, DDX10 is remarkably increased in HCC tissues in fresh tissues. In addition, cellular function assays demonstrated that DDX10 promotes cell viability, proliferation. Furthermore, we validated that up-regulated DDX10 enhances the activity of Wnt/beta-catenin signaling to promote cell proliferation.