Biomarker Study of Primary Nonmetastatic versus Metastatic Invasive Bladder Cancer' the Cutoff Value for P53 Nuclear Overexpression Was 20%

A cohort of 109 patients with primary transitional cell carcinomas, stages T2-T3, grade 2 or higher, was identified and further divided into two groups based on lymphatic metastasis at the time of cystectomy (n = 57 cases) or absence of detectable metastatic disease over a minimum of 5 years of follow-up after cystectomy (n = 52). Blocks corresponding to the primary tumor lesions were sectioned and distributed to different laboratories to be analyzed. Immu-nohistochemistry on deparaffinized tissue sections was conducted for evaluation of p53 nuclear overexpression (mono-clonal antibody PAb!801), assessment of proliferative index (Ki-67 antigen-monoclonal antibody MIB1), and microvas-cular counts (factor YHI-related antigen). DNA content/ ploidy studies were performed on material obtained from thick sections. A double-blinded strategy was used for the evaluation of laboratory data versus clinical parameters. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with I 8 U.S.C. Section 1734 solely to indicate this fact.