Study of the Bio-Tolerance of Aqueous extract of ( Balanophoraceae ) in Isa Brown Laying Hens

This experiment was conducted to evaluate the effect of aqueous extract of Thonningia sanguinea parameters and organs of Isa Brown laying hens. To do this, 220 laying hens of 16 weeks old (1113 ± 90 g lively mass) were randomly divided into two (02) groups of 110 hens each. The hens of group II (treated group) were treated with THOS week (07 days) at a rate of 10g/L while the hens of group I (control group) received only water without any additives. Blood samples were taken at day 0 (D0) (before treatment), D3 (third day of treatment) and D8, D14, D21 and D28 (after treatment) for determination of some biochemical and hematological parameters. Furthermore six hens in each group were slaughtered on day 8 (D8), D14, D21 and on D28. Vital organs (heart, liver, lungs, kidneys, spleen, duodenum, thymus and bone marrow) were then removed for histopathological analysis. THOS supplementation had no significant effect on hematological parameters as well as on the activity of serum enzymes such as lowered the activity of ALP and LDH. Compared to th Na + ; Cl ; Ca 2+ ; Mg 2+ but significantly decreased potassium (K creatinine, bilirubin, total protein and uric acid), THOS has not metabolism but it has led to a significant decrease in triglycerides levels at D3 and D21. However, total cholesterol and tot lipids levels have not undergone significant change. The analysis of histo change, nor tissue damage. These results suggest that supplementation with THOS is well tolerated by laying hens organism and that its use would cause no major danger for the survival of laying hens