Isolation and Identification of a New Bacterial Strains Degrading Paracetamol Isolated from Yemeni Environment

Paracetamol has emerged as an important environment pollutants due to its wide usage. This study carried out to isolate and identify of a novel paracetamol-degrading bacteria from the contaminated site of pharmaceutical wastewater at Sana’a City, Yemen. The enrichment media and selective media were used to isolate and purify the bacteria from wastewater samples. The isolated bacteria were identified according to phenotypic and genotypic characterization using 16S rRNA sequence analysis. The results showed that the three bacterial strains and signed as an STB1, STB3 and STB5. The STB1 strain was identified as Pseudomonas aeruginosa strain DSM 50071 with similarity 92%, while the STB3 and STB5 were identified as P. aeruginosa strain NBRC 12689 with similarity 89% and 99%, respectively. The optimal conditions for paracetmol degradation by all stains were recorded at 30 ̊C and pH 7.0. Also, the paracetamol degradation was found to be influenced by an increase in the bacterial cell concentration. The STB1, STB3, and STB5 strains were degraded 68.6%, 51.3% and 79.4% of 4000 mg/L paracetamol, respectively, in 120h. The results performed that the degradation potential of the three isolated bacterial strains possibly played a major active role in the paracetamol removal processes. Citation: Wadhah H Edrees., et al. “Isolation and Identification of a New Bacterial Strains Degrading Paracetamol Isolated from Yemeni Environment”. Clinical Biotechnology and Microbiology 1.6 (2018): 257-270. Volume 1 • Issue 6 • 2018 Page 257 to 270 Volume 1 Issue 6 January 2018 © All Copy Rights are Reserved by Wadhah H Edrees., et al. Clinical Biotechnology and Microbiology Paracetamol or acetaminophen is a common analgesic and an anti-inflammatory commonly used as a non-prescription drug and sold as over-the-counter drug worldwide [1]. The paracetamol consumption has increased throughout the world in the last decades. In England, it was consumed as one of the top three drugs prescribed, while in the USA, it was consumed as one of the top 200 prescriptions [2,3]. It was ranked as the second prescription drugs throughout the year 2008 in Kuwait [4]. In Yemen, it was also ranked as first of top ten drugs produced by local industries and one of the top ten drugs imported [5]. Introduction ISSN: 2575-4750