Silencing of TIPE 3 suppresses proliferation of human glioma cells via AKT signaling pathway

TIPE3, a member of TNFAIP8 (tumor necrosis factor-alpha-induced protein 8, or TIPE) family, was reported to promote tumorigenesis by acting as a transfer protein of lipid second messenger. Glioma is the most common and lethal brain tumor in central nervous system. However, the role of TIPE3 on glioma remains unknown. In the present study, siRNA interference was performed to silence TIPE3 expression. CCK-8, colony formation assay, EdU incorporation assay and flow cytometry analysis were employed to examine glioma cell proliferation and cell cycle after TIPE3 knockdown. Western blot was used to investigate the expression of AKT, p-AKT, cyclinD1, p21 and pHistoneH3. The results showed that silencing of TIPE3 inhibited glioma cell proliferation and caused cell cycle arrest. TIPE3 depletion suppressed activity of phosphorylation AKT pathway consistent with down-regulating cyclinD1 expression and elevating p21 levels. Taken together, our study indicates down-regulation of TIPE3 inhibits glioma cell proliferation via PI3K/AKT pathway. Moreover, our study suggests that TIPE3 may act as a therapeutic target for the treatment of glioma.