Ferrous ion-chelating Assay for analysis of antioxidant potential of Sargassum sp and Iyengaria sp

Aim: To analyse antioxidant potential of Sargassum sp and lyengaria sp by using ferrous ion chelating assay. Methods: Sargassum sp and Iyengaria sp were collected from southeast coast of Karachi. The powdered seaweed samples (500g of Sargassum sp and Iyengaria sp) were extracted with methanol in conical flasks at room temperature for three weeks. The samples were filtered using Whatman filter paper to obtain clarified filtrates. Ferrous ion-chelating assay was used for evaluation of antioxidant potential. Results: The methanolic extract of Sargassum sp showed higher potential as evidenced by Ferrous ion-chelating Assay. Conclusion: This is a preliminary study highlighting potential of Sargassum sp and future studies must converge on in-vitro analysis of these species for a better understanding of the biological mechanisms regulated by Sargassum sp to control cellular activities.