Intervention Effect of Ethanol Extract of Hedyotis Diffusa and Gefitinib on the Epithelial Mesenchymal Transition Process of Human Lung Adenocarcinoma Cell H 358 Induced By TGF-β 1

OBJECTIVE To investigate the ethanol extract of Hedyotis diffusa(EEHD) and gefitinib(Gef) used on the human lung adenocarcinoma H358 cell, which has been induced to epithelial mesenchymal transition by transforming growth factor beta 1(TGF-β1) in vitro. METHODS The H358 cells were successfully induced to the epithelial mesenchymal transformation model by 5 ng·mL TGF-β1 for 24 h. After preparation of the EEHD, respectively added EEHD for 48 h(Group A), Gef for 48 h(Group B), EEHD for 24 h and then Gef for 24 h(Group A24B24), Gef for 24 h and then EEHD for 24 h(Group B24A24), EEHD combined with Gef for 48 h(Group AB) and the control group to the model cells. Then the rates of cell growth were detected by CCK-8 test, the protein expression of epithelial marker (E-cadherin) and mesenchymal cell marker (Vimentin) were detected by Western-blot. And the rates of apoptosis were tested by flow cytometry. RESULTS In all other groups, the 基金项目:浙江省医药卫生科技计划项目(2013KYB187);浙江省中医药科技计划项目(2013ZQ014) 作者简介:吕昕,女,硕士,主治医师 Tel: (0571)86620305 E-mail: LX1206@163.com