Malathion induced changes in catalase and superoxide dismutase in testicular tissues of goat in vitro

In the present study alterations in the activity of two antioxidant enzymes, catalase (EC.1.15.1.1) and superoxide dismutase (EC.1.11.1.6) in Malathion exposed testicular tissue of goat in vitro have been analyzed by spectrophotometric method. Malathion exposure significantly decreased catalase activity from 2.29±0.05 to 1.66±0.05 and 1.49±0.03 after 4 hour of exposure at 1nm and 100nm/ml concentration respectively. After 8 hour exposure at 100nm/ml showed significant decline from 2.13±0.16 to 1.33±0.04.At the same dose level, Malathion induced a significantly decline in superoxide dismutase activity from 6.96±0.15 to 4.85±0.20, 4.12±0.12 and 3.84±0.04 after 8 hour of exposure (p<0.05). These results indicate that short-term exposure to Malathion induces oxidative stress in the testis by decreasing antioxidant enzymes.